HLA-E and HLA-G dCODE Dextramer®
For analysis of antigen-specific NK and T cells
Next-Generation Sequencing and Single-cell Multi-omics are powerful methods to deeply characterize mucosal-associated invariant T cells (MAIT) cells with information on ligand specificity, surface protein and gene expression along with TCR sequences.
With an optimized number of human MR1-ligand complexes, PE fluorophores, and a unique DNA barcode, MR1 dCODE Dextramer® reagents enable comprehensive analysis of mucosal-associated invariant T (MAIT) cells by single-cell multi-omics.
Whether MR1 Monomers, MR1 Dextramer® or MR1 dCODE Dextramer® reagents, Immudex offers two ligands, preloaded into the MR1 Monomer:
Custom ligand-MR1 specificities can be requested through Custom Solutions and Services.
The unique DNA barcode of MR1 dCODE Dextramer® seamlessly integrates into the BD Rhapsody™ or 10x Chromium workflow allowing you to couple the ligand specificity with surface marker expression, gene expression, and TCR sequence data for a deeper understanding of MAIT cells and their complex biology.
In a proof of concept study, we analyzed the TCRs of 171 CD3+ CD161+ 5-OP-RU-specific MAIT cells using MR1 dCODE Dextramer®. Intriguingly, the majority of the analyzed TCRs belonged to the TRAV V1-2*01 subtype and generally exhibited low variability.
Learn more about compatibility with the BD Rhapsody™ or 10x Chromium platforms.
Immudex provides flexibility to your MAIT cell research by offering biotinylated Monomers and MR1 Dextramer® reagents in addition to MR1 dCODE Dextramer® technology for your assay of choice.
Mouse MR1 reagents and custom ligand-MR1 complexes can be requested through Custom Solutions and Services.
MR1 dCODE Dextramer® technology provides better research tools to:
Select ligand-specific TCRs for MAIT cell-based therapy using MR1 Dextramer® or MR1 dCODE Dextramer® reagents:
Goals
MR1 dCODE Dextramer® staining and library preparation follows the same process as for dCODE Dextramer®. Find the protocols on our protocol page.
To order MR1 Products, please send an e-mail to [email protected] specifying information on:
Please note: MR1 dCODE Dextramer® is available as a standard product with the PE fluorochrome, or with FITC or APC fluorochromes (or no fluorochrome) via Custom Solutions and Services.
Product |
MR1 |
Ligand |
Label |
Amount |
MR1 dCODE Dextramer® |
Human MR1 |
5-OP-RU |
PE |
25 / 50 /150 tests* |
MR1 dCODE Dextramer® |
Human MR1 |
6-FP |
PE |
25 / 50 /150 tests* |
*Higher amounts or test sizes are available upon request.
Do you have questions about the MR1 dCODE Dextramer® technology, implementing our technology into your experimental workflow, protocol optimization, or data analysis and interpretation?
Our technical support team consists of highly skilled scientists that are here to empower you to improve your results, provide you with the tools for success, and share our expertise.